With significant developments in the areas of chromatography and spectroscopy as well as the unique inherent chemical diversity of natural products, vital in drug research, natural products research has gained new momentum. Fully updating and adding to the previous two editions, Natural Products Isolation, Third Edition documents the latest methods and technologies for natural products isolation with a combination of all new chapters and revised and expanded classic methods. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Natural Products Isolation, Third Edition provides the substantial background information needed by budding natural product researchers as well offering an invaluable reference guide to available methodologies and techniques for the more experienced researchers.
An Introduction to Natural Products Isolation.- Initial and Bulk Extraction of Natural Products Isolation.- Supercritical Fluid Extraction in Natural Products Analyses.- Accelerated Solvent Extraction (ASE®) for Natural Products Isolation.- Microwave-Assisted Extraction in Natural Products Isolation.- An Introduction to Planar Chromatography and Its Application to Natural Products Isolation.- Isolation of Natural Products by Low-Pressure Column Chromatography.- Isolation of Natural Products by Ion-Exchange Methods.- Separation of Natural Products by Countercurrent Chromatography.- Isolation of Natural Products by Preparative High Performance Liquid Chromatography (Prep-HPLC).- Isolation of Natural Products by Preparative Gas Chromatography.- Hyphenated Techniques and Their Applications in Natural Products Analysis.- Extraction of Plant Secondary Metabolites.- Isolation of Marine Natural Products.- Isolation of Microbial Natural Products.- Extraction and Isolation of Saponins.- Extraction and Isolation of Phenolic Compounds.- Scaling-Up of Natural Products Isolation.- Follow-Up of Natural Products Isolation.- Natural Products Isolation in Modern Drug Discovery Programs.
