Now in its eighth edition, Principles of Gene Manipulation and Genomics embraces the burgeoning revolution in recombinant DNA technology and its applications. Providing integrated coverage of the techniques used for gene manipulation, genomics, and its related disciplines, the text features full-color illustrations throughout. Chapter summaries and thought-provoking end-of-chapter questions plus a dedicated website provides further instruction and resources for both the student and instructor as well as regular updates on important topics elucidate learning for undergraduate and graduate courses in genetics, genomics, genome analysis, and gene cloning understanding.
List of plates. Preface. Abbreviations. 1 Gene manipulation in the post-genomics era. Part I Fundamental Techniques of Gene Manipulation. 2 Basic techniques. 3 Cutting and joining DNA molecules. 4 Basic biology of plasmid and phage vectors. 5 Cosmids, phasmids, and other advanced vectors. 6 Gene-cloning strategies. 7 Sequencing genes and short stretched of DNA. 8 Changing genes: site-directed mutagenesis and protein engineering. 9 Bioinformatics. Part II Manipulating DNA in Microbes, Animals, and Plants. 10 Cloning in bacteria other than Escherichia coli. 11 Cloning in Saccharomyces cerevisiae and other fungi. 12 Gene transfer to animal cells. 13 Genetic manipulation of animals. 14 Gene transfer to plants. 15 Advanced transgenic technology. Part III Genome Analysis, Genomics, and Beyond. 16 The organization and structure of genomes. 17 Mapping and sequencing genomes. 18 Comparative genomics. 19 Large-scale mutagenesis and interference. 20 Analysis of the transcriptome. 21 Proteomics I Expression analysis and characterization of proteins. 22 Proteomics II Analysis of protein structures. 23 Proteomics III Protein interactions. 24 Metabolomics and global biochemical networks. Part IV Applications of Gene Manipulations and Genomics. 25 Applications of genomics: understanding the basis of polygenic disorders and identifying quantitative trait loci. 26 Applications of recombinant DNA technology. Glossary. References. Index
